The average survival rate of patients with malignant brain gliomas(GBM) is about 6-11months and this study was designed to find a new way to treat these patients.
Copper ion is essential cofactor of angiogenesis and its lead to proliferation of endothelial cells therefore copper depletion inhibits the angiogenic response induced by human brain tumor cells.The study population is patients with glioblastoma multiforme(GBM) aged between 18-65 years in both male and female sex in two intervention and control group and each one consisted of 25 patients.At treatment day,patients based on the main criteria included pathological diagnosis; tumor recurrence; age equal or greater than 18; KPS 60 or more and literacy are classified. in the intervention group, a low copper diet and penicillamine with initial dose of 250 mgr and increased to 1gr and in control group placebo at the same dose will be administered. For control of diet, both groups assess by food frequency questionnaire at the beginning, middle and end of the study. Drug and diet compliance also will be controlled by call. Serum copper concentration will be measured in the time of Baseline and after a month and a half (center study) and at the end of three months (end of study).As the primary outcome factors include VEGF, IL-6, IL-8 , TNF-α and KPS in both groups will be measured at the beginning and end of the study. To investigate the probable side effects of intervention, hemoglobin, WBC, platelet count, creatinine, bilirubin, liver function tests, PT and PTT measure in the beginning, middle and end of the study. Intervention period is considered for 3 months.