Objective: detection effect of Black tea and Green tea consumption on salivary PH.
This analytical study is Clinical Trial.
Inclusion criteria: 20-22 Years old healthy people who have consent to enter the study. People with a history of black tea consumption.
Exclusive criteria: systemic disease, acute or chronic infection and dental or periodontal abscess, salivary gland disorders, history of radiation therapy, smoking, addiction, pregnancy.
The statistical population consisted of 50 volunteers from Zahedan Dental School students. In this study, all volunteers will be invited to consume black tea on the first day and after collecting saliva samples (in the manner described below), it will be replaced with Green tea (3-4 cups per day) and after that, in the second day before and after consuming Green tea samples will be collected again.
For sampling, on the first day (8-9 A.M) we will ask each volunteer, before consuming Black tea, trickle their unstimulated saliva into the test tubes through his lower lip within 3 minutes and every 60 seconds (in seated position) and after half an hour recollect the samples 1, 5 and 10 minutes after ingestion of 250 ml Black tea without any sweetener in separate tubes (For each volunteer, test tubes numbered 1 to 4) and it will be transferred to laboratory to evaluate salivary PH. Then ask volunteers to replace Black tea with Green tea on that day.
On the second day (8-9 A.M) we will ask each volunteer, before consuming Green tea, trickle their unstimulated saliva into the test tubes through his lower lip within 3 minutes and every 60 seconds (in seated position) and after half an hour recollect the samples 1, 5 and 10 minutes after ingestion of 250 ml Green tea without any sweetener in separate tubes (For each volunteer, test tubes numbered 1 to 4) and it will be transferred to laboratory to evaluate salivary PH.
In the laboratory amount of salivary pH will be measured by digital PH-meter.