Inclusion criteria: age about 35 to 65 years old, Having no anatomic disorder in face, Wrinkle type between grade 2 to 4, Normal mental health, Ability to comply with the study requirements, Provide written informed consent and comply with the study requirements, Negative pregnancy test (Females)/ Exclusion criteria: being smokers or drinkers any past skin intervention in face, Having many wrinkles on the face type of 1 or 5 of wrinkle scoring, having a mental disorder, Previous treatment with the sponsor's product, History of active autoimmune disease or organ transplantation, Diagnosis of cancer, unless successfully treated or in remission (basal cell carcinoma is excluded), Active or chronic skin disease, Known genetic disorders affecting fibroblasts or collagen, Active systemic infection Requires chronic antibiotic or steroidal therapy, Use of certain commercial products/procedures to the treatment area prior to study enrollment or plans for use during the study Known allergic reactions to agents used in preparation of treatment, Excessive exposure to sun without adequate sun protection, Use of systemic agents that increase bleeding or clotting, or disorders equated with these effects