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Protocol summary

Study aim
Evaluation of safety and efficacy of intra-articular transplantation of allogeneic adipose-derived mesenchymal stem cells on pain relieving and physical activity improvement, in patients with knee joint osteoarthritis.
Design
A triple-blind randomized controlled clinical trial phase I/II
Settings and conduct
First we will recruit 3 patients with osteoarthritis admitted in orthopedic clinic of shafa yahyaiean hospital evaluating safety of allogeneic adipose derived mesenchymal stem cells, and if there were no serious side effects after 3 months, another 90 patients in two groups: case group (allogeneic adipose derived MSCs) and control group (normal saline) will be studied in terms of their positive effects in pain reduction and improvement in physical function.
Participants/Inclusion and exclusion criteria
Patients with 18 to 65 years old Whose arthritis is detected with MRI and are not affected with chronic diseases or cancer, are recruited.
Intervention groups
Intra-articular transplantation of 100 millions of allogeneic adipose-derived mesenchymal stromal cells in case group and injection of 3-5 ml NL/S in control group
Main outcome variables
evaluation of allogeneic adipose derived MSCs transplantation on pain and patient’ physical activity and the thickness of affected cartilage

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20080728001031N23
Registration date: 2018-04-24, 1397/02/04
Registration timing: registered_while_recruiting

Last update: 2021-08-26, 1400/06/04
Update count: 1
Registration date
2018-04-24, 1397/02/04
Registrant information
Name
Nasser Aghdami
Name of organization / entity
Royan Institute
Country
Iran (Islamic Republic of)
Phone
+98 21 2356 2000
Email address
nasser.aghdami@royaninstitute.org
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2017-03-14, 1395/12/24
Expected recruitment end date
2019-03-21, 1398/01/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Evaluation of safety and efficacy of intra-articular transplantation of allogeneic adipose derived mesenchymal stromal cells in patients with knee joint osteoarthritis An interventional randomized triple-blind placebo controlled clinical trial phase I/II
Public title
Allogeneic stem cell transplantation in knee joint osteoarthritis
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Patients in the range of 18 to 65 years old osteoarthritis diagnosed by MRI
Exclusion criteria:
Pregnancy or lactating Positive tests for HIV, HCV, HBV Active central or peripheral neurologic disorder End organ damage or Uncontrolled endocrine disorder Active respiratory disorder and reaction to sedative drugs history of cancer
Age
From 18 years old to 65 years old
Gender
Both
Phase
1-2
Groups that have been masked
  • Participant
  • Care provider
  • Investigator
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 100
Randomization (investigator's opinion)
Randomized
Randomization description
Randomized allocation to intervention and control groups
Blinding (investigator's opinion)
Triple blinded
Blinding description
Participants would be allocated in case and control groups according to a confidential table of accidental numbers which will be just available for the lab responsible. Care provider, investigator and outcome assessor are not aware of the substance which injected. In order to eliminate data analyzer's errors, group types would not be uncovered until the results are determined.
Placebo
Used
Assignment
Parallel
Other design features
Block randomization

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Royan infertility institute
Street address
Eastern Hafez St,Northern Banihashem St, 45 m Resalat highway
City
Tehran
Province
Tehran
Postal code
1665659911
Approval date
2017-10-10, 1396/07/18
Ethics committee reference number
IR.ACECR.ROYAN.REC.1396.138

Health conditions studied

1

Description of health condition studied
cell therapy in osteoarthritis
ICD-10 code
M15.9
ICD-10 code description
Polyosteoarthritis, unspecified

Primary outcomes

1

Description
Side effects
Timepoint
One week, one month, 3, 6 and 12 months after transplantation
Method of measurement
Physical examination, adverse effect questionnaire

2

Description
Joint function
Timepoint
Before, 3, 6 and 12 months after transplantation
Method of measurement
WOMAC and KOOS questionnaires for osteoarthritis

Secondary outcomes

1

Description
Cartilage thickness
Timepoint
Before and 12 months after transplantation
Method of measurement
MRI

2

Description
Molecular and cellular indexes (IL-6, IL-10, TNF-α, IFN-γ, COMP, CTX-II, HA, CS846, CD4, CD3, CD8, CD25)
Timepoint
Before, one week, 3 and 6 months after transplantation
Method of measurement
Blood test

3

Description
Subchondral edema
Timepoint
Before and 12 months after transplantation
Method of measurement
MRI

4

Description
Joint effusion
Timepoint
Before and 12 months after transplantation
Method of measurement
MRI

5

Description
new cartilage formation
Timepoint
Before and 12 months after transplantation
Method of measurement
MRI

Intervention groups

1

Description
Phase I and case group in phase II: intraarticular transplantation of single dose 100 millions of allogeneic adipose derived mesenchymal stem cells
Category
Other

2

Description
Control group (phase II): intraarticular transplantation of single dose 3 to 5 ml normal saline as placebo
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Orthopedic Clinic of Shafa Yahyaeian hospital
Full name of responsible person
Dr. Abolfazl Bagheri fard
Street address
Mojahedin e eslam street, Baharestan Square
City
Tehran
Province
Tehran
Postal code
۱۴۴۹۶۱۴۵۳۵
Phone
+98 21 3354 2006
Email
bagherifard@gmail.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Royan Institute
Full name of responsible person
Abdol hossein Shahverdi
Street address
Eastern Hafez St,Northern Banihashem St, 45 m Resalat highway
City
Tehran
Province
Tehran
Postal code
1665659911
Phone
+98 21 2356 2000
Email
shahverdi@royaninstitute.org
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Royan Institute
Proportion provided by this source
90
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

2

Sponsor
Name of organization / entity
Shafa Yahyaeian Hospital
Full name of responsible person
Abolfazl Bagheri fard
Street address
Mojahedin e eslam street, Baharestan Sq
City
Tehran
Province
Tehran
Postal code
۱۴۴۹۶۱۴۵۳۵
Phone
+98 21 3354 2001
Email
bagherifard@gmail.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Shafa Yahyaeian Hospital
Proportion provided by this source
10
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Royan cell therapy center
Full name of responsible person
Shahedeh Karimi Torshizi
Position
M.D/ Researcher
Latest degree
Medical doctor
Other areas of specialty/work
Orthopedics
Street address
Eastern Hafez St, Northern Banihashem St, 45 m Resalat highway
City
Tehran
Province
Tehran
Postal code
1665659911
Phone
+98 21 2356 2000
Email
karimi58shah@gmail.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Royan Institute
Full name of responsible person
Nasser Aghdami
Position
M.D,Ph.D
Latest degree
Ph.D.
Other areas of specialty/work
Immunology
Street address
Royan Institute, Eastern Hafez St,Northern Banihashem St, Resalat highway 45 meters
City
Tehran
Province
Tehran
Postal code
1665659911
Phone
+98 21 2356 2000
Email
nasser.aghdami@royaninstitute.org

Person responsible for updating data

Contact
Name of organization / entity
Royan Institute
Full name of responsible person
Hoda Madani
Position
Researcher/M.D
Latest degree
Medical doctor
Other areas of specialty/work
Cardiology
Street address
Royan Institute, Eastern Hafez St,Northern Banihashem St, 45 m Resalat highway
City
Tehran
Province
Tehran
Postal code
1665659911
Phone
+98 21 2356 2000
Email
hoda.madani@royaninstitute.org

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Not applicable
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Not applicable
Analytic Code
Not applicable
Data Dictionary
Not applicable
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