Protocol summary

Study aim
Safety of 3 Times Intravenous Transplantation of َAllogeneic Different Sources Derived-Mesenchymal Stem Cells in ARDS Patients
Design
A uncontrolled, parallel, double-blind, randomized, clinical trial, phase II 4 groups, 3 patients in each group, totally 12 patients 4 cell interventional groups 12 months follow-up
Settings and conduct
We carry out a randomized, double-blind phase II trial of 3 times Intravenous infusion of allogeneic mesenchymal stem cells derived from different sources for treatment in 12 ARDS patients, 3 bone marrow derived MSC, 3 adipose derived MSC, 3 wharton's jelly derived MSC and 3 amniotic membrane derived MSC, in a 1:1:1:1 randomization. Each patient receives 3 Intravenous infusions every other day. Patients will be followed daily during 7 days after the first transplantation, Then at 2 week, 4 weeks, 3, 6 and 12 months. Three doses of 200×10#6 cells are intravenously infused as a naturally dropped single dose over 15-20 minutes in every other day. The cell products will be supplied by CELLTECH company and Royan institute. The products will be transferred to Baqiyatallah hospital and transplanted.
Participants/Inclusion and exclusion criteria
• Aged between 18 to 65 years • Male or female • Signed informed consent voluntarily • Confirmed ARDS
Intervention groups
First Intervention group: Wharton's jelly derived mesenchymal stem cells Second Intervention group: Amniotic membrane derived mesenchymal stem cells Third Intervention group: Bone marrow derived mesenchymal stem cells Forth Intervention group: Adipose derived mesenchymal stem cells
Main outcome variables
Numbers of Patients Occurred Any Unexpected Severe Adverse Events (Including All-cause Deaths)

General information

Reason for update
Acronym
Allo-MSCs-ARDS
IRCT registration information
IRCT registration number: IRCT20080901001165N44
Registration date: 2020-03-28, 1399/01/09
Registration timing: prospective

Last update: 2020-03-28, 1399/01/09
Update count: 0
Registration date
2020-03-28, 1399/01/09
Registrant information
Name
Yunes Panahi
Name of organization / entity
Baqiyatallah University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 21 8821 1524
Email address
yunespanahi@bmsu.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2020-03-29, 1399/01/10
Expected recruitment end date
2020-08-31, 1399/06/10
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
A Comparison Study on Safety & Efficacy of Repeated Intravenous Infusion of Allogeneic Mesenchymal Stem Cell from Different Sources in ARDS Patients: A Randomized, Double Blind, Clinical Trial Phase II
Public title
A Comparison Study of Repeated Intravenous Infusion of Allogeneic Mesenchymal Stem Cell from Different Sources in ARDS Patients
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Signed informed consent voluntarily Confirmed ARDS SOFA score between 2-3 point PaO2/FIO2 ≤ 300 mmHg Mild to Moderate pneumonia/ stay in the ICU <48 hours Pulmonary imaging shows that the focus progress > 50% in 24-48 hours
Exclusion criteria:
Co-Infection of HIV, tuberculosis, influenza virus, adenovirus and other respiratory infection virus Liver or kidney SOFA score of more than 3 points; combined with other organ failure Patients with malignant tumor Pregnant or lactating women Uncontrolled underling diseases Pulmonary obstructive pneumonia, severe pulmonary interstitial fibrosis, allergic alveolitis, and other known viral pneumonia or bacterial pneumonia In vitro life support (ECMO, ECCO2R, RRT)
Age
From 18 years old to 65 years old
Gender
Both
Phase
1-2
Groups that have been masked
  • Participant
  • Care provider
  • Outcome assessor
Sample size
Target sample size: 12
Randomization (investigator's opinion)
N/A
Randomization description
Blinding (investigator's opinion)
Double blinded
Blinding description
There are the same cell bags for infusion during study. Clean room staff, DSMB and data statistician just aware of their contain. So, the mentioned people (clinicians for assessment, health care system staff for infusion and patients) will not inform about the type of intervention.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
National Institute for Medical Research Development
Street address
No. 21, Besat Ave, West Fatemi Ave, Tehran
City
Tehran
Province
Tehran
Postal code
۱۴۱۹۶۹۳۱۱۱
Approval date
2020-03-25, 1399/01/06
Ethics committee reference number
IR.NIMAD.REC.1399.002

Health conditions studied

1

Description of health condition studied
Acute respiratory distress syndrome (ARDS)
ICD-10 code
J80
ICD-10 code description
Acute respiratory distress syndrome

Primary outcomes

1

Description
Numbers of Patients Occurred Any Unexpected Severe Adverse Events (Including All-cause Deaths)
Timepoint
Before Intervention, Day 0 (First Intervention) till Day 7, Day 14, Day 28, 3 Moths, 6 Months and 12 months after intervention
Method of measurement
Fill Common Terminology Criteria for Adverse Events (CTCAE) Form

Secondary outcomes

1

Description
PaO2/FiO2 assessment
Timepoint
Before Intervention, Day 0 (First Intervention) till Day 7, Day 14, Day 28, 3 Moths, 6 Months and 12 months after intervention
Method of measurement
Ventilator

Intervention groups

1

Description
First Intervention group: Wharton's jelly derived mesenchymal stem cells
Category
Treatment - Drugs

2

Description
Second Intervention group: Amniotic membrane derived mesenchymal stem cells
Category
Treatment - Drugs

3

Description
Third Intervention group: Bone marrow derived mesenchymal stem cells
Category
Treatment - Drugs

4

Description
Forth Intervention group: Adipose derived mesenchymal stem cells
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Baghiyyatollah hospital
Full name of responsible person
Dr. Mostafa Ghanei
Street address
Mollasadra Ave, after Sheikh Bahaei cross, Vanak square, Tehran
City
Tehran
Province
Tehran
Postal code
1435915371
Phone
+98 21 8805 0435
Email
mghaneister@gmail.com
Web page address
https://baq.bmsu.ac.ir/Portal/Home/

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Bagheiat-allah University of Medical Sciences
Full name of responsible person
Dr.Mostafa Ghanei
Street address
Mollasadra Ave, after Sheikh Bahaei cross, Vanak square, Tehran
City
Tehran
Province
Tehran
Postal code
1435915371
Phone
+98 21 8805 0435
Email
mghaneister@gmail.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
No
Title of funding source
National Institute for Medical Research Development
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Other

Person responsible for general inquiries

Contact
Name of organization / entity
Iranian academic center for education culture and research
Full name of responsible person
Hoda Madani
Position
Co-PI
Latest degree
Medical doctor
Other areas of specialty/work
Medical Biology
Street address
No. 9, Shaghayegh Deadend, Banihashem St, Resalat Ave, Tehran
City
Tehran
Province
Tehran
Postal code
1665664511
Phone
+98 21 2251 8388
Email
hoda62_m@yahoo.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Bagheiat-allah University of Medical Sciences
Full name of responsible person
Mostafa Ghanei
Position
professor
Latest degree
Subspecialist
Other areas of specialty/work
Internal Medicine
Street address
Mollasadra Ave, after Sheikh Bahaei cross, Vanak square, Tehran
City
Tehran
Province
Tehran
Postal code
1435915371
Phone
+98 21 8805 0435
Email
mghaneister@gmail.com

Person responsible for updating data

Contact
Name of organization / entity
Bagheiat-allah University of Medical Sciences
Full name of responsible person
Mostafa Ghanei
Position
Professor
Latest degree
Subspecialist
Other areas of specialty/work
Internal Medicine
Street address
Mollasadra Ave, after Sheikh Bahaei cross, Vanak square, Tehran
City
Tehran
Province
Tehran
Postal code
1435915371
Phone
+98 21 8805 0435
Email
mghaneister@gmail.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
No - There is not a plan to make this available
Statistical Analysis Plan
No - There is not a plan to make this available
Informed Consent Form
No - There is not a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
No - There is not a plan to make this available
Data Dictionary
No - There is not a plan to make this available
Title and more details about the data/document
Case Report Form
When the data will become available and for how long
Start of access period 6 months after printing results
To whom data/document is available
Researchers working in academic and scientific institutions
Under which criteria data/document could be used
complementary studies related to cell products
From where data/document is obtainable
Email to corresponding author
What processes are involved for a request to access data/document
Reply to email by corresponding author (1 week) After confirmation, data files will be sent less than two weeks
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