Effect of short-term garlic supplementation in two different dose on total antioxidant capacity and some markers of serum oxidative stress in rest and induced-exercise exhaustive in soccer players

Introduction: Vigorous exercise-induced generation of free radicals lead to oxidative damage in biological macromolecules such as proteins, DNA and lipids. One way to confront the adverse effects of oxidative stress caused by intense exercises is to consume dietary antioxidant supplementation such as garlic. Considering anti-oxidative effects of garlic and its combinations on oxidative stress caused by doing intense exercises, only a few studies have been carried out in this regard. Therefore, this study an attempt to determine the effects of Shuttle run exercise test and short-term garlic supplementation (14 days) on serum Total Antioxidant Capacity (TAC), oxidative stress marker (malondialdehyde, MDA) and cellular damage (Creatine Kinase, CK) in soccer men. Methods: This study will carry out in double-blind quasi-experimental designs on Thirty soccer men (aged 18-23 years,55-65 ml/kg/min and BMI 20-22 kg/m2) who will divid into three random and equal groups: supplement groups (1200 and 2400 mg/day garlic) and placebo group (dextrose). Before and after supplementation period (14-days), all subjects participat in the Shuttle run exhaustive physical activity. Blood samples will taken in four phases: before and immediately after first exercise test (Before14-day supplementation); and before and immediately after first exercise test (after 14-day supplementation).The serum total antioxidant capacity (TAC) and Malondialdehyde (MDA) will be determined by FRAP and spectrophotometer, respectively. Creatine kinase changes will be measured by automatic analyzer. The normal data will analyzed by repeated measure ANOVA, Bonferroni and independent t test at α≤0.05.

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