Protocol summary

Study aim
The aim of this study is to evaluate the effect of oral rinse of povidine iodine and hydrogen peroxide mouthwash in comparison with normal saline on viral load in confirmed covid 19 patients who may undergo dental treatment.
Design
this double blinde clinical trial having control group and parallel groups and randomized phase2 in 60 participants . block randomization method using the online RANDOM.ORG software was done.
Settings and conduct
The target population will be consisted of patients of both sex who are hospitalized in shahid faghihi hospital, Namazi hospital and Aliasghar hospital, Shiraz University Of Medical Sciences, Shiraz, IRAN due to Covid -19 disease. All the participants, the nurse which will do the sampling and the author will be blinded from each other.
Participants/Inclusion and exclusion criteria
The inclusion criteria are age 18-50,and the hospitalized confirmed covid 19 patients. Exclusion crireria are having any systemic disease or medications that may interact with Povidine Iodine and-or Hydrogen Peroxide, patients who are not feeling well, not cooperative patients, and those who are not willing to participate in the study
Intervention groups
In each group two saliva samples is needed. first at 0 hour (before gargle), and the second, 5 to 10 minutes after using mouthwashes. Group A (n=20) patients on 10 ml gargle using 0.25% Povidone-Iodine, for 20-30 seconds. Group B (n=20) patients will be subjected to 10 ml gargle of 1% Hydrogen peroxide for 20-30 seconds. Group C (n=20) will serve as positive control. These will be given simple normal saline gargle for 20-30 seconds,
Main outcome variables
To determine the viral load before and after the intervention.

General information

Reason for update
Acronym
SARS-COV2, Covid19
IRCT registration information
IRCT registration number: IRCT20201212049681N1
Registration date: 2021-02-28, 1399/12/10
Registration timing: registered_while_recruiting

Last update: 2021-02-28, 1399/12/10
Update count: 0
Registration date
2021-02-28, 1399/12/10
Registrant information
Name
zahra rajabzadeh
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 71 3631 5271
Email address
dr.zrajabzadeh@gmail.com
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2021-01-20, 1399/11/01
Expected recruitment end date
2021-03-19, 1399/12/29
Actual recruitment start date
2021-01-20, 1399/11/01
Actual recruitment end date
2021-03-19, 1399/12/29
Trial completion date
2021-04-19, 1400/01/30
Scientific title
Evaluating the effect of gargling with Hydrogen Peroxide 1% and Povidine Iodine 0.25% on viral load of SARS-CoV-2 in covid19 patients ; A pilot randomized clinical trial
Public title
Evaluating the effect of gargling with Hydrogen Peroxide 1% and Povidine Iodine 0.25% on viral load of SARS-CoV-2 in covid19 patients
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
having age 18-50, those who are hospitalized and have confirmed covid 19 viral contamination.
Exclusion criteria:
having any systemic disease or medications that may interact with Povidine Iodine and-or Hydrogen Peroxide patients who are not feeling well, not cooperative patients, and those who don’t want to participate in the study
Age
From 18 years old to 50 years old
Gender
Both
Phase
3
Groups that have been masked
  • Participant
  • Care provider
  • Investigator
  • Outcome assessor
Sample size
Target sample size: 60
More than 1 sample in each individual
Number of samples in each individual: 2
1 sample before intervention and one sample after intervention
Actual sample size reached: 60
Randomization (investigator's opinion)
Randomized
Randomization description
The patients Will be equally divided into three experimental groups, namely, the A,B,C group. The allocation of the participants will be based on the block randomization method using the online RANDOM.ORG software. Each random number will be placed in a sealed opaque envelope and subsequently, each participant randomly will pick one envelope corresponding to either the A,B, orC group.
Blinding (investigator's opinion)
Double blinded
Blinding description
To ensure both the participants and clinicians will be blinded to the clinical trial,all the participants, the nurse which will do the sampling and the author will be blinded from each other, and are not aware of the mouthwash that is used.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Shiraz University Of Medical Sciences
Street address
Department of Endodontics, School of Dentistry, Ghasrdast Street, Shiraz, Iran,
City
Shiraz
Province
Fars
Postal code
71956-15878
Approval date
2021-01-09, 1399/10/20
Ethics committee reference number
IR. SUMS. DENTAL.REC.1399.186

Health conditions studied

1

Description of health condition studied
Covid-19
ICD-10 code
RAO1.0
ICD-10 code description
severe acute respiratory syndrome corona virus

Primary outcomes

1

Description
viral load before the intervention, viral load after the intervention
Timepoint
before intervention and 5-10 minutes after intervention
Method of measurement
real-time reverse transcriptase polymerase chain reaction (rRT-PCR)

Secondary outcomes

1

Description
viral transmission in saliva
Timepoint
before the intervention and 5-10 minutes after the intervention
Method of measurement
real-time reverse transcriptase polymerase chain reaction (rRT-PCR)

Intervention groups

1

Description
intervention group: Group A (n=20) patients on 10 ml gargle using 0.25% Povidone-Iodine, for 20-30 seconds,
Category
Treatment - Drugs

2

Description
intervention group: Group B (n=20) patients will be subjected to 10 ml gargle using 1% Hydrogen peroxide for 20-30 seconds.
Category
Treatment - Drugs

3

Description
Control group: Group C (n=20) will serve as positive control. These will be given simple normal saline gargles for 20-30 seconds,
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Shahid Faghihi hospital
Full name of responsible person
Mahdi Sedigh Shams
Street address
Department of Endodontics, School of Dentistry, Ghasrdast Street, Shiraz, Iran
City
Shiraz
Province
Fars
Postal code
71956-15878
Phone
+98 71 3832 3435
Email
adla@sums.ac.ir
Web page address
https://sums.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Shiraz University of Medical Sciences
Full name of responsible person
Azadeh Andishe tadbir
Street address
: Department of Endodontics, School of Dentistry, Ghasrdast Street, Shiraz, Iran,
City
Shiraz
Province
Fars
Postal code
71956-15878
Phone
+98 71 3832 3435
Email
adla@sums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Shiraz University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Shiraz University of Medical Sciences
Full name of responsible person
Zahra Rajabzadeh
Position
resident
Latest degree
Medical doctor
Other areas of specialty/work
Dentistry
Street address
Department of Endodontics, School of Dentistry, Ghasrdast Street, Shiraz, Iran,
City
Shiraz
Province
Fars
Postal code
71956-15878
Phone
+98 71 3832 3435
Email
adla@sums.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Shiraz University of Medical Sciences
Full name of responsible person
Zahra Rajabzadeh
Position
resident
Latest degree
Medical doctor
Other areas of specialty/work
Dentistry
Street address
Department of Endodontics, School of Dentistry, Ghasrdast Street, Shiraz, Iran,
City
Shiraz
Province
Fars
Postal code
71956-15878
Phone
+98 71 3832 3435
Email
adla@sums.ac.ir

Person responsible for updating data

Contact
Name of organization / entity
Shiraz University of Medical Sciences
Full name of responsible person
Zahra Rajabzadeh
Position
Student
Latest degree
Medical doctor
Other areas of specialty/work
Dentistry
Street address
Ghasrodasht avenue
City
Shiraz
Province
Fars
Postal code
71956-15878
Phone
+98 71 3631 5271
Fax
Email
dr.zrajabzadeh@gmail.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Yes - There is a plan to make this available
Data Dictionary
Not applicable
Title and more details about the data/document
the main outcome information
When the data will become available and for how long
starting 6 months after the publication
To whom data/document is available
academic and industrial investigators
Under which criteria data/document could be used
academic and industrial investigators
From where data/document is obtainable
adla@sums.ac.ir
What processes are involved for a request to access data/document
after the investigation
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