Protocol summary

Study aim
Evaluation of the effect of synbiotic supplementation on clinical manifestations, inflammatory and non-inflammatory markers in hospitalized Covid-19 patients compared with placebo
Design
Clinical trial with control group (placebo), with parallel groups, double-blind, randomly blocked, phase 3 on 60 patients. The www.sealedenvelope.com was used to generate a blocked random allocation sequence.
Settings and conduct
This study is a randomized clinical trial and patients admitted with Covid 19 in Ghaem Hospital in Mashhad, after obtaining informed consent, patients are randomly placed in one of two intervention groups or placebo. Double-blind randomization will be done by packets in the package. According to the pre-designed checklist, patients in both groups will be followed up for 14 days from the time of admission, and clinical signs, inflammatory and non-inflammatory markers will be taken from patients on the first day and the fourteenth day of follow-up.
Participants/Inclusion and exclusion criteria
Inclusion criteria: Conscious consent Hospitalized patients with Covid 19 Age category 18 years and above Exclusion criteria: Pregnancy and lactation Hospitalization in the ICU
Intervention groups
In the intervention group, patients, take two synbiotic daily for 14 days. In the placebo group, patients take two placebo daily for 14 days.
Main outcome variables
Levels of inflammatory factors CRP, ESR and IL-6 as well as ALT, AST, ALP, CBC, and Creatinine on the first and fourteenth day of follow-up. Evaluation of clinical symptoms such as cough, fever, rapid breathing, sore throat, whole body pain, shortness of breath, SPO2 with and without oxygen, weakness and lethargy, abdominal pain, chest pain and gastrointestinal symptoms (diarrhea, vomiting and nausea) Daily until the end of the follow-up.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20210531051459N1
Registration date: 2021-10-02, 1400/07/10
Registration timing: registered_while_recruiting

Last update: 2021-10-02, 1400/07/10
Update count: 0
Registration date
2021-10-02, 1400/07/10
Registrant information
Name
Mona Kabiri
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 51 3841 7403
Email address
kabirimn@mums.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2021-09-27, 1400/07/05
Expected recruitment end date
2022-04-25, 1401/02/05
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of Lactocare synbiotic on clinical manifestations, inflammatory and non-inflammatory markers in hospitalized covid-19 patients
Public title
The effect of synbiotic in hospitalized covid-19 patients
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Hospitalized patients with definitive diagnosis of Covid 19 using PCR or CT scan Conscious consent to participate in the study Age category 18 years and above
Exclusion criteria:
Acute pancreatitis Pregnancy and lactation Having autoimmune diseases and taking immunosuppressants or drugs used to reject transplants Taking supplements containing probiotics and prebiotics in the last three months Hospitalization in the ICU Patients treated with herbal medicines or other traditional medicine methods Dialysis patients Having a history of allergies to synbiotics Dissatisfaction with participating in the study
Age
From 18 years old
Gender
Both
Phase
3
Groups that have been masked
  • Participant
  • Care provider
  • Outcome assessor
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
Randomization type: Block Randomization unit: individual Randomization tool: Random number table using www.sealedenvelope.com How to create a random sequence: At www.sealedenvelope.com, the randomization section, after selecting create a list, specifies the number of groups, block sizes and list length, and accordingly, presents the list randomization. Allocation Concealment: Sealed envelopes
Blinding (investigator's opinion)
Double blinded
Blinding description
In this study, participants did not know the type of treatment they received. Also, patient clinicians, physicians, and outcome assessors are unaware of how patients are grouped and use medication or placebo.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Mashhad University of Medical Sciences
Street address
Ghaem Hospital, Ahmad Abad Ave., Mashhad
City
Mashhad
Province
Razavi Khorasan
Postal code
٩٩١٩٩-٩١٧۶۶
Approval date
2021-08-03, 1400/05/12
Ethics committee reference number
IR.MUMS.MEDICAL.REC.1400.338

Health conditions studied

1

Description of health condition studied
COVID-19
ICD-10 code
U07.1
ICD-10 code description
U07.1 COVID-19, virus identified

Primary outcomes

1

Description
The level of CRP inflammatory marker
Timepoint
At the beginning of the study and fourteen days after taking the capsule
Method of measurement
Blood test

2

Description
The level of IL-6 inflammatory cytokine
Timepoint
At the beginning of the study and fourteen days after taking the capsule
Method of measurement
ELISA

3

Description
ESR level
Timepoint
At the beginning of the study and fourteen days after taking the capsule
Method of measurement
Blood test

Secondary outcomes

1

Description
ALT level
Timepoint
At the beginning of the study and fourteen days after taking the capsule
Method of measurement
Blood test

2

Description
AST level
Timepoint
At the beginning of the study and fourteen days after taking the capsule
Method of measurement
Blood test

3

Description
ALP level
Timepoint
At the beginning of the study and fourteen days after taking the capsule
Method of measurement
Blood test

4

Description
Creatinine level
Timepoint
At the beginning of the study and fourteen days after taking the capsule
Method of measurement
Blood test

5

Description
Complete Blood Count (CBC)
Timepoint
At the beginning of the study and fourteen days after taking the capsule
Method of measurement
Blood test

6

Description
Cough
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

7

Description
Fever
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

8

Description
Breathing rate
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up (number of breaths per minute)

9

Description
Sore throat
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

10

Description
Generalized body pain
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

11

Description
Dyspnea
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

12

Description
SPO2
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

13

Description
Weakness and lethargy
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

14

Description
stomach pain
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

15

Description
Chest pain
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

16

Description
Gastrointestinal symptoms (diarrhea, vomiting and nausea)
Timepoint
Daily (first day to fourteenth day of intervention)
Method of measurement
Clinical check up

Intervention groups

1

Description
Intervention group: In the intervention group, hospitalized patients with Covid 19, in addition to standard treatment (Remdesivier, and glucocorticoids such as dexamethasone, methylprednisolone, and prednisolone), take two supplements of Lactocarb synobiotic daily after meals for 14 days. Lactocare capsules contains beneficial and safe bacterial strains along with prebiotic fructooligosaccharide. Lactocar capsule made by Zist Takhmir Company is gluten free and its CFU is 10^9.
Category
Treatment - Drugs

2

Description
Control group: In the control group (placebo), hospitalized patients with Covid 19 in addition to standard treatment (Remdesivier, and glucocorticoids such as dexamethasone, methylprednisolone, and prednisolone), take two placebo daily after meals for 14 days. The placebo is exactly the same color, shape, weight, and packaging as the Lactocare capsule. Placebo capsules are also purchased from Zist Takhmir Company.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Ghaem hospital
Full name of responsible person
Mona Kabiri
Street address
Ghaem hospital, Ahmad Abad Ave.
City
Mashhad
Province
Razavi Khorasan
Postal code
٩٩١٩٩-٩١٧۶۶
Phone
+98 51 3841 7403
Fax
Email
Kabirimn@mums.ac.ir
Web page address
https://quaem.mums.ac.ir/

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Mashhad University of Medical Sciences
Full name of responsible person
Dr. Mohsen Tafaghodi
Street address
Vice Chancellor for Research and Technology, Daneshgah Ave.
City
Mashhad
Province
Razavi Khorasan
Postal code
9138813944
Phone
+98 51 3841 1538
Email
vcresraech@mums.ac.ir
Web page address
https://v-research.mums.ac.ir/
Grant name
Grant code / Reference number
992359
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Mashhad University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Mashhad University of Medical Sciences
Full name of responsible person
Mona Kabiri
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Medical Nanotechnology
Street address
Clinical Research Development Unit, First Floor, Narjes building, Ghaem hospital, Ahmad Abad Ave.
City
Mashhad
Province
Razavi Khorasan
Postal code
٩٩١٩٩-٩١٧۶۶
Phone
+98 51 3841 7403
Email
kabirimn@mums.ac.ir
Web page address
https://crdc.mums.ac.ir/

Person responsible for scientific inquiries

Contact
Name of organization / entity
Mashhad University of Medical Sciences
Full name of responsible person
Mona Kabiri
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Medical Nanotechnology
Street address
Clinical Research Development Unit, First Floor, Narjes building, Ghaem hospital, Ahmad Abad Ave.
City
Mashhad
Province
Razavi Khorasan
Postal code
٩٩١٩٩-٩١٧۶۶
Phone
+98 51 3841 7403
Email
Kabirimn@mums.ac.ir
Web page address
https://crdc.mums.ac.ir/

Person responsible for updating data

Contact
Name of organization / entity
Mashhad University of Medical Sciences
Full name of responsible person
Mona Kabiri
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Medical Nanotechnology
Street address
Clinical Research Development Unit, First Floor, Narjes building, Ghaem hospital, Ahmad Abad Ave.
City
Mashhad
Province
Razavi Khorasan
Postal code
٩٩١٩٩-٩١٧۶۶
Phone
0098518417403
Email
Kabirimn@mums.ac.ir
Web page address
https://crdc.mums.ac.ir/

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
No - There is not a plan to make this available
Informed Consent Form
No - There is not a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
No - There is not a plan to make this available
Data Dictionary
No - There is not a plan to make this available
Title and more details about the data/document
The potential data can be shared after unidentified individuals.
When the data will become available and for how long
Access period starts 9 months after the published results
To whom data/document is available
The data will be available to researchers working in academic and scientific institutions.
Under which criteria data/document could be used
The use of data and its analysis is allowed by mentioning the source.
From where data/document is obtainable
Email the author of the article to receive the data.
What processes are involved for a request to access data/document
The processes that researcher who request data go through will include a letter of request from the person, a letter of request from the center or university of origin, and acceptance of the destination university to receive the information.
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