Eight weeks of MRT (Metabolic Resistance Training) and CGA (Colorogenic Acid) consumption on BMP 2, 4, 6, 7 (Bones Morphogenic Proteins) and Ucp1 (Un Coupling Protein 1) protein expression and HDL ( High density Lipoprotein) in overweight women

Effect of Eight weeks of metabolic resistance training and green coffee consumption on the expression of bone morphogenic proteins and the expression of nonplacental proteins and serum high-density lipoprotein in overweight women.

A clinical trial with a control group without blinding and with block randomization. Randomization was done in two levels to determine the intervention of exercise and the intervention of supplement.

During the study, female candidates for the test will refer to Walia Elah Clinic in Tehran, and if they are eligible, they will be included in the study and will be randomly assigned to the intervention and control groups using the random block method. Blinding was not done in this study

Inclusion criteria: Body Mass Index (BMI) less than 28 and more than 25, being physically active, not taking medicine or supplements, Respiratory health, Having a Covid 19 vaccine card. Exclusion criteria: pregnancy, menopause, and addiction to alcohol or tobacco.

Intervention group 1: 400 mg green coffee supplement by Sahle company three times a day for two months; Intervention group 2: Metabolic Resistance Training three days a week for 45 minutes for eight weeks; Intervention group 3: 400 mg green coffee supplement along with Training three days a week for 45 minutes during eight weeks; Control group: Without training and taking green coffee supplements

Expression of bone morphogenic proteins (2 4 6 7 ) and uncoupled proteins and serum high density lipoprotein

In this study, the block randomization method will be used. Randomization will be done in two levels to determine the exercise intervention and then to determine the intervention of the supplement. First, using the letters A (intervention) and B (no-intervention), 6 blocks of 4 are created. Each letter must be repeated twice in each block (example: AABB). Each of the created blocks is given a number from 1 to 6 and then one of the blocks of 4 is selected using a dice. The dice will be thrown a total of ten times to determine the status of all participants in the study. The order of the letters in the selected block will indicate the status of training intervention for the people participating in the study. Each time the dice is thrown, the status of the training intervention will be determined for 4 consecutive people participating in the study. After the end of this phase, the people participating in the study will be divided into two groups of 20, including the training intervention group and the control group. Then again, the block randomization method will be used according to the previous steps to determine the type of supplement intervention. This stage will be done separately for people in the supplement intervention and no-intervention groups. At this stage, the letter A will indicate receiving nutritional supplements and B will indicate no intervention. Finally, after the completion of this stage, there will be 40 double combinations of letters A and B (with the possibility of repetition), and these combinations will be numbered from 1 to 40, and each number will have a random 8-digit code of a combination of letters and numbers. The determined combinations are written on paper and placed in a closed envelope. Then the code related to them is written on the envelope and all envelopes are given to the research team. After the first person enters the study, the code of the envelope (8-digit code) is announced to the group of researchers and they are asked to assign the person to one of the four groups according to the letters inside the envelope (the first letter indicates the status of training intervention and the second one indicates the status of nutritional supplement intervention, for example, a person with AB envelope will be assigned to the training intervention group and no- intervention supplement group). This process will continue until the arrival of the 40th person, and the research team will not be aware of the allocation status of the next patient and the envelope associated with the next patient during sampling.