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Protocol summary

Study aim
The effect of probiotic administration on severity of the menopausal symptoms and mental health of the menopausal women
Design
Clinical trial with control group, with parallel groups, triple blind, randomized
Settings and conduct
Participants are randomly assigned to the two groups of 45 recipients of Probiotic capsule and placebo group.This study will be conducted in Comprehensive Health Centers of Hamadan city. In this study, neither the researcher, nor the menopausal women and nor the analyzer knows the contents of the package and triple blinded will be performed. In intervention group, Probiotic capsule and in Control group, placebo will be prescribed Once a day for 6 weeks.
Participants/Inclusion and exclusion criteria
Inclusion criteria: age between 45-55, having menopause symptoms; not using cigarette or alcohol, not having physical or mental diseases, not using of relaxation techniques, not using of hormonal or herbal therapies Exclusion criteria: irregular use of the drug, refuse from continuing to participate in study
Intervention groups
Intervention group: Probiotic supplement will be prescribed in the form of Oral Capsule 200 mg, Once a day for 6 weeks. This drug Will be Producted by Hamadan University of Medical Sciences. Control group: placebo will be prescribed in the form of Oral Capsule contain ُof Starch 200 mg Once a day for 6 weeks. The placebo is made in Hamadan University of Medical Sciences
Main outcome variables
Severity of menopause symptoms Stress-anxiety-depression

General information

Reason for update
Having menopause symptoms
Acronym
IRCT registration information
IRCT registration number: IRCT20180707040370N9
Registration date: 2023-01-19, 1401/10/29
Registration timing: prospective

Last update: 2024-08-16, 1403/05/26
Update count: 2
Registration date
2023-01-19, 1401/10/29
Registrant information
Name
Ensiyeh Jenabi
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 81 3838 0496
Email address
e.jenabi@umsha.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2023-02-20, 1401/12/01
Expected recruitment end date
2023-08-23, 1402/06/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of probiotic administration on severity of the menopausal symptoms and mental health of the menopausal women
Public title
The effect of probiotic administration on severity of the menopausal symptoms and mental health
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
age between 45-55 not using cigarette or alcohol not having physical or mental diseases not using of relaxation techniques not using of hormonal or herbal therapies at least 1 to 5 years have passed since menopause Having menopause symptoms
Exclusion criteria:
irregular use of the drug refuse from continuing to participate in study
Age
From 45 years old to 55 years old
Gender
Female
Phase
2
Groups that have been masked
  • Participant
  • Investigator
Sample size
Target sample size: 90
Randomization (investigator's opinion)
Randomized
Randomization description
Participants are randomly selected by random.org computer program that are assigned to the two groups of 45 recipients of capsule Probiotic and placebo. The allocation of the participants to the groups is performed by method of allocation concealment. In this way, using the random block 4, the allocation sequence is specified. Then, in the sealed envelopes, the intervention name is written in sequential order, and is numbered accordingly
Blinding (investigator's opinion)
Triple blinded
Blinding description
The drug packages are similar and were given to menopausal women by other person. This person does not inform about the package contents. Therefore, neither the researcher, nor the menopausal women and nor the analyzer knows the contents of the package and triple blinding will be performed.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics Committee of Hamadan University of Medical Sciences
Street address
Hamadan University of Medical Sciences, Fahmiheh Street, Hamadan
City
Hamadan
Province
Hamadan
Postal code
6517838678
Approval date
2023-01-18, 1401/10/28
Ethics committee reference number
IR.UMSHA.REC.1401.887

Health conditions studied

1

Description of health condition studied
Severity of the Menopausal Symptoms and Mental health
ICD-10 code
N95.1
ICD-10 code description
Menopausal and female climacteric states

Primary outcomes

1

Description
Severity of menopause symptoms
Timepoint
Before the intervention, 3 and 6 weeks after the intervention
Method of measurement
MRS scale

2

Description
Stress
Timepoint
Before the intervention, 3 and 6 weeks after the intervention
Method of measurement
Questionnaire DASS-21

3

Description
anxiety
Timepoint
Before the intervention, 3 and 6 weeks after the intervention
Method of measurement
Questionnaire DASS-21

4

Description
depression
Timepoint
Before the intervention, 3 and 6 weeks after the intervention
Method of measurement
Questionnaire DASS-21

Secondary outcomes

empty

Intervention groups

1

Description
Intervention group: Probiotic supplement will be prescribed in the form of Oral Capsule 200 mg, Once a day for 6 weeks. Including Lactobacillus plantarum, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve and Streptococcus thermophilus.This drug Will be Producted by Hamadan University of Medical Sciences
Category
Treatment - Drugs

2

Description
Control group: placebo will be prescribed in the form of Oral Capsule contain ُof Starch 200 mg Once a day for 6 weeks. The placebo is made in Hamadan University of Medical Sciences
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Comprehensive Health Service Center of Shohada
Full name of responsible person
Ensiyeh Jenabi
Street address
Comprehensive Health Service Center of Shohada., Takhti Ave., Hamadan
City
Hamadan
Province
Hamadan
Postal code
6517838344
Phone
+98 81 3252 4321
Email
Shohada.health@yahoo.com

2

Recruitment center
Name of recruitment center
Comprehensive Health Service Center of Khezr
Full name of responsible person
Ensiyeh Jenabi
Street address
Comprehensive Health Service Center of Khezr., Khezr Ave., Hamadan
City
Hamadan
Province
Hamadan
Postal code
6517838289
Phone
+98 81 3266 0550
Email
Khezr.Health@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Hamedan University of Medical Sciences
Full name of responsible person
Saeid Bashirian
Street address
Vice-chancellor for Research, Hamadan University of Medical Sciences, Shahid Fahmideh Av
City
Hamadan
Province
Hamadan
Postal code
6517838695
Phone
+98 81 3838 0717
Email
S_bashrian@yahoo.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
No
Title of funding source
En Hamedan University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Hamedan University of Medical Sciences
Full name of responsible person
Ensiyeh Jenabi
Position
Assistant professor
Latest degree
Ph.D.
Other areas of specialty/work
Gynecology and Obstetrics
Street address
Vice-chancellor for Research, Hamadan University of Medical Sciences, Shahid Fahmideh Ave
City
Hamadan
Province
Hamadan
Postal code
6517838695
Phone
+98 81 3838 0496
Email
en.jenabi@yahoo.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Hamedan University of Medical Sciences
Full name of responsible person
Ensiyeh Jenabi
Position
Assistant Professor
Latest degree
Ph.D.
Other areas of specialty/work
Gynecology and Obstetrics
Street address
Vice-chancellor for Research, Hamadan University of Medical Sciences, Shahid Fahmideh Ave
City
Hamadan
Province
Hamadan
Postal code
6517838695
Phone
+98 81 3838 0496
Email
en.jenabi@yahoo.com

Person responsible for updating data

Contact
Name of organization / entity
Hamedan University of Medical Sciences
Full name of responsible person
Ensiyeh Jenabi
Position
Assistant Professor
Latest degree
Ph.D.
Other areas of specialty/work
Gynecology and Obstetrics
Street address
Vice-chancellor for Research, Hamadan University of Medical Sciences, Shahid Fahmideh Ave
City
Hamadan
Province
Hamadan
Postal code
6517838695
Phone
+98 81 3838 0496
Email
en.jenabi@yahoo.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
No - There is not a plan to make this available
Informed Consent Form
No - There is not a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
No - There is not a plan to make this available
Data Dictionary
Not applicable
Title and more details about the data/document
A portion of the information, such as information on the main outcome or the like, can be shared
When the data will become available and for how long
6 months after publishing the results
To whom data/document is available
Researchers working in academia
Under which criteria data/document could be used
Data analysis is permitted with the author's permission.
From where data/document is obtainable
Hamadan ،Vice-chancellor for Research, Hamadan University of Medical Sciences, Shahid Fahmideh Ave Responsible person: Ensiyeh Jenabi Postal code: 6517838695 Phone number: +98 81 3838 0496 Email: en.jenabi@yahoo.com
What processes are involved for a request to access data/document
Requests should be submitted to the corresponding author via email. He will follow up with the inquiry
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