Effect of low-calorie Dietary Approaches to Stop Hypertension diet (DASH) and synbiotic supplement on metabolic risk factors, serum levels of some inflammatory, oxidant factors and antimullerin hormone in women with polycystic ovary syndrome

Determining the combined effect of low-calorie Dietary Approaches to Stop Hypertension diet (DASH) and synbiotic supplement on metabolic risk factors, serum levels of some inflammatory factors, oxidants, and antimullerin hormone in women with polycystic ovary syndrome

The clinical trial has four groups, including three intervention groups and one control group, with parallel, double-blind, randomized, phase 3 groups on 76 patients. A table of random numbers will be used for randomization.

This study will be conducted as a double-blind clinical trial on 76 women aged 18 to 45 in Ahvaz for 12 weeks. The study subjects will be randomly allocated in one of the following four groups: 1- receiving low-calorie Dietary Approaches to Stop Hypertension diet (DASH) + synbiotic supplement (500 mg per day) 2- receiving low-calorie DASH diet + placebo 3- receiving standard low-calorie diet + synbiotic supplement (500 mg per day) 4- receiving standard low-calorie diet + placebo (control group) Lack of knowledge of the participants and the examiners of the results of the characteristics of the group of people

Age between 18 and 45 years Body mass index between 27-35 Diagnosis and confirmation of polycystic ovary syndrome by an endocrinologist based on the Rotterdam criteria

Four studied groups 1- receiving low-calorie Dietary Approaches to Stop Hypertension diet (DASH) + synbiotic supplement (500 mg per day) 2- receiving low-calorie DASH diet + placebo 3- receiving standard low-calorie diet + synbiotic supplement (500 mg per day) 4- receiving standard low-calorie diet + placebo (control group)

CRP, HBA1C, GSH, AMH, insulin, leptin, testosterone, fasting glucose, TG, HDL-C, TC, LDL-C, visceral obesity index, insulin resistance, insulin sensitivity, AIP and VAI, malondialdehyde

In this study, blocked randomization method will be used. In this method, even-sized blocks are created, and in these blocks, a quarter of the people in each block will be considered as belonging to one of the four groups. Then, among the created blocks, so many blocks are randomly selected to reach the required sample size.

People participating in the study will be studied randomly in one of four groups. The supplement and placebo will be given to people with the same appearance and packaging, so that the people under study and the researcher will not know about the contents of the packages. Finally, the person who will do the evaluation of the results will not know how to allocate people.