The effect of 8 weeks of aerobic exercise and astaxanthin supplementation on humanin protein expression, miR-17, miR-146a and miR-155, and inflammatory conditions, oxidative stress and apoptosis in women with type 2 diabetes

Investigating the effect of 8 weeks of combined training with astaxanthin supplementation on the expression of HN protein, miR-17, miR-155, miR-146a, inflammation, oxidative stress and apoptosis in type 2 diabetic patients.

Clinical trial with control group, with parallel groups, single-blind, randomized

The exercises are done for 8 weeks (3 sessions per week). Training groups get to know the environment and exercises before starting sports programs. Also, the consent form for participating in the research will be obtained from all the participants.

Patients will be excluded from the study if they have any of the following conditions: a history of ketoacidosis within 6 months before enrollment in the study, excessive hyperglycemia (HbA1c > 10%), diabetic microvascular complications, diabetic neuropathy, impaired liver function ( plasma aminotransferase and/or γ-glutamyltransferase levels above the upper limit of normal for age and sex), impaired renal function (serum creatinine levels above the normal range for age and sex), severe anemia, uncontrolled hypertension (hypertension > 100/170 mmHg). Patients with cardiovascular disease (class I-IV congestive heart failure or history of myocardial infarction), obvious heart-related symptoms or history of cerebrovascular events will also be excluded. Active patients who reported more than 1 exercise per week and patients on insulin therapy will not be eligible.

Five groups of aerobic exercise (15 people), aerobic exercise with placebo (15 people), aerobic exercise with astaxanthin supplement (15 people), astaxanthin supplement use (15 people) and control group with placebo (15 people) were divided.

Humanin serum levels; antioxidant capacity; serum levels of antioxidant enzymes; TNF-α; IL-10; BAX؛ BCL2 ؛ miR-17؛ miR-146؛ miR-155

Simple randomization method; The law of random assignment is the simplest method of limited randomization. This method represents a large block for the entire sample size, which means that the balance in the number of people assigned to each group will be achieved at the end of the study. For this purpose, the researcher first determined a total sample size, then randomly assigned groups of them to group A and the rest to group B. For example, in a study with a sample size of 200 people, 100 balls for intervention groups A and 100. The ball for intervention group B is placed in a lottery container, and then the balls are randomly removed from the container without replacement, and the created sequence is recorded.

The participants in the design and the person who performs the data analysis are unaware of the study group assignment (which group received the supplement and which group received the placebo).

All data is potentially shareable after de-identifying individuals

The access period starts 6 months after the results are published

داده های شما فقط برای محققین شاغل در موسسات دانشگاهی و علمی در دسترس خواهد بود

Use in meta-analysis articles

To receive the desired documents or data, refer to the email of Dr. Keyvan Khorramipour.

Six months after the time of publication of the results in a valid journal, in case of additional research work