Investigating the possibility of producing a beneficial non-alcoholic grape drink containing parapsychobiotics with the ability of reducing stress

In the current research, lactobacillus plantarum paraprobiotic cells have been used in Shahani grape drink to reduce stress in graduate students. ​

In order to investigate the effect of a drink containing parapsychobiotics and removing the effect of grape juice on stress, a comparative method was used. This study was a double-blind clinical trial with a design of two groups before and after. The sample size was calculated based on Almada et al.'s study (10). They were tested to evaluate the effect of two types of drinks. In order to investigate and compare the effect of drinks, the amount of changes in stress-related indicators in the states of each of the consumers and their saliva before and after using the drink were measured qualitatively and quantitatively.

In order to investigate the effect of a drink containing parapsychobiotics and removing the effect of grape juice on stress, a comparative method was used. Each consumer was asked to consume 250 ml of the drink daily for 10 weeks. The place of one of the universities of Tehran province.

The criteria for entering the test include the company's consent, age between 20 and 30 years, not consuming alcohol, not suffering from various neurological, cardiac, blood pressure, and diabetes disorders, and not taking drugs that affect stress and the nervous system, and systolic blood pressure within the normal range.

Based on the monitoring, 32 students were selected. These 32 people were divided into 2 groups. In fact, in this research, the main variable is the type of drink and the secondary variable (adjusting) is the gender of the consumers.

Salivary cortisol level, sleep quality level, general health, delay in falling sleep

Participants: Although the participants were fully aware of the study and filled the consent form, they were not aware of the type of drink consumed. Clinical Caregiver: The person who was in contact with the control and treatment users answered their questions without knowing the drink consumed by each person. Evaluator: The evaluator collected the forms filled by the volunteers without knowing the drink consumed by each person. Data analysis: The person analyzing the data analyzed the data without knowing which person the collected data belongs to.