Short-term effect of vitamin D supplementation on superoxide dismutase (SOD) andglutathione peroxidase (GPX) following a high-intensity interval training session in malevolleyball players
Investigating the short-term effect of vitamin D supplementation on superoxide dismutase (SOD) and glutathione peroxidase (GPX) after a high-intensity interval training session in male volleyball players.
Design
The design of the present study is a clinical trial with a control group, single blinding, and randomization. The randomization process will utilize the rand function of Excel software to ensure that the assignment of participants to the control and experimental groups is done in a randomized manner.
Settings and conduct
The present study will be conducted at the Faculty of Sports Sciences of Razi University of Kermanshah. The study is a cross-sectional study and single blinding.
Participants/Inclusion and exclusion criteria
Entry Criteria
Male volleyball players aged 16-20 will be eligible for entry into the study.
Non-Entry Criteria
The study will likely exclude individuals outside the specified age range and those not actively engaged in high-intensity interval training. Additionally, individuals with specific medical conditions or taking certain medications may be excluded from participation in the study.
Intervention groups
The intervention groups in this study will consist of male volleyball players aged 16-20, who will be divided into two experimental groups and a control group. The experimental group will receive vitamin D supplementation for 4 weeks, while the control group will receive a placebo instead of vitamin D. Both groups will undergo two sessions of high-intensity interval training to evaluate the short-term effect of vitamin D supplementation and placebo on the levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in their bodies.
Short-term effect of vitamin D supplementation on superoxide dismutase (SOD) andglutathione peroxidase (GPX) following a high-intensity interval training session in malevolleyball players
Public title
Short-term effect of vitamin D supplementation on superoxide dismutase (SOD) andglutathione peroxidase (GPX)
Purpose
Other
Inclusion/Exclusion criteria
Inclusion criteria:
Age and Gender: Participants must be male volleyball players within a specific age range to ensure consistency in the study group.
Vitamin D Levels: Participants must have a defined baseline level of vitamin D to establish a consistent starting point for the study.
Training Experience: Participants should have a minimum level of training experience or proficiency in high-intensity interval training to ensure they can perform the exercise regimen effectively and safely.
Health Status: Participants must be in good health and free from any conditions that could affect the study results, such as chronic illnesses or vitamin deficiencies unrelated to the study parameters.
Exclusion criteria:
Existing Vitamin D Supplementation: Individuals who are already taking vitamin D supplements would not be eligible for the study to prevent confounding effects from existing supplementation.
History of Chronic Diseases: Participants with a history of chronic diseases, such as diabetes or autoimmune disorders, may be excluded due to the potential impact of these conditions on the study outcomes.
Recent Antioxidant Use: Individuals who have recently used antioxidant supplements or medications that could affect SOD and GPX levels may be excluded to avoid interference with the study results.
Injury or Illness: Individuals who have suffered recent injuries or illnesses that could affect their ability to participate in high-intensity interval training may be excluded to ensure the safety and consistency of the exercise regimen.
Age
From 16 years old to 20 years old
Gender
Male
Phase
N/A
Groups that have been masked
Participant
Sample size
Target sample size:
28
Randomization (investigator's opinion)
Randomized
Randomization description
In this study, randomization will involve the unbiased and unpredictable assignment of male volleyball players to different groups. Each participant will have an equal chance of being assigned to either the group receiving vitamin D supplementation or the control group receiving a placebo or no supplementation. This process will help to minimize the influence of confounding variables and biases, ensuring that the comparison between the groups accurately reflects the impact of the supplementation on SOD and GPX levels following high-intensity interval training. Randomization will be achieved using methods such as computer-generated random numbers or randomization tables, and it will be a critical component of rigorous experimental design to draw valid conclusions from the study results.
Blinding (investigator's opinion)
Single blinded
Blinding description
In the this study, blinding will be implemented to prevent bias and ensure the integrity of the research. To achieve this, the participants involved in the study will be kept unaware of whether the male volleyball players are receiving vitamin D supplementation or a placebo. This single-blind approach will involve the administration of supplements in identical-looking packaging, with only researcher retaining the information regarding the actual contents of the supplements.
Placebo
Used
Assignment
Other
Other design features
Secondary Ids
empty
Ethics committees
1
Ethics committee
Name of ethics committee
Ethics committee of Kermanshah Razi University
Street address
No.9, Taqh-Bostan Ave, University Blvd, Razi University.
City
Kermanshah
Province
Kermanshah
Postal code
6714967346
Approval date
2024-01-17, 1402/10/27
Ethics committee reference number
IR.RAZI.REC.1402.097
Health conditions studied
1
Description of health condition studied
Antioxidant indicators
ICD-10 code
ICD-10 code description
Primary outcomes
1
Description
Antioxidant capacity
Timepoint
before the beginning and at the end of the study
Method of measurement
Blood sampling
Secondary outcomes
empty
Intervention groups
1
Description
Intervention group: Fourteen subjects received vitamin D supplementation, including vitamin D from Zahrawi Pharmaceutical Company, at a dosage of 5000 units per day for four weeks. The test involved high-intensity interval training on a treadmill for two sessions: one session before supplementation and one session after supplementation. The intensity of exercise was monitored using a Polar heart rate monitor and Borg's index, with blood samples taken on four occasions. The first and second stages of sampling occurred before and after the first training protocol, while the third and fourth stages of sampling took place after completing the second training protocol.
Category
Lifestyle
2
Description
Control group: Fourteen subjects received a placebo containing paraffin manufactured by Zahrawi Pharmaceutical Company in Iran daily for four weeks. The placebo was designed to resemble a vitamin D supplement in terms of shape, color, smell, and taste. The test involved high-intensity interval training on the treadmill for two sessions: one session before receiving the placebo and one session after its completion. The intensity of the exercise was monitored using a Polar heart rate monitor and the Borg index, with blood samples taken on four occasions. The first and second stages of sampling occurred before and after the first training protocol, while the third and fourth stages of sampling took place after completing the second training protocol.
Category
Lifestyle
Recruitment centers
1
Recruitment center
Name of recruitment center
آزمایشگاه فیزیولوژی ورزشی دانشکده علوم ورزشی دانشگاه رازی کرمانشاه
Full name of responsible person
Hidar Ihsan Murad
Street address
No.9, Taqh-Bostan Ave, University Blvd, Razi University.
City
Kermanshah
Province
Kermanshah
Postal code
6714967346
Phone
+98 83 3427 7605
Fax
+98 83 3427 7606
Email
hayderihsan91@gmail.com
Sponsors / Funding sources
1
Sponsor
Name of organization / entity
Razi University
Full name of responsible person
رستگار حسینی
Street address
No.9, Taqh-Bostan Ave, University Blvd, Razi University.
City
Kermanshah
Province
Kermanshah
Postal code
6714967346
Phone
+98 83 3427 7605
Fax
+98 83 3427 7606
Email
R.hoseini@razi.ac.ir
Grant name
Razi University
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
No
Title of funding source
Razi University
Proportion provided by this source
10
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic
Person responsible for general inquiries
Contact
Name of organization / entity
Razi University
Full name of responsible person
Nasser Behpour
Position
Associate Professor
Latest degree
Ph.D.
Other areas of specialty/work
Physiology
Street address
No.9, Taqh-Bostan Ave, University Blvd, Razi University.
City
Kermanshah
Province
Kermanshah
Postal code
۶۷۱۴۴۱۴۹۷۱
Phone
+98 83 3427 6505
Email
n_behpoor@yahoo.com
Person responsible for scientific inquiries
Contact
Name of organization / entity
Razi University
Full name of responsible person
Nasser Behpour
Position
Associate Professor
Latest degree
Ph.D.
Other areas of specialty/work
Physiology
Street address
No.9, Taqh-Bostan Ave, University Blvd, Razi University.
City
Kermanshah
Province
Kermanshah
Postal code
6714414971
Phone
0833427605
Email
n_behpoor@yahoo.com
Person responsible for updating data
Contact
Name of organization / entity
Razi University
Full name of responsible person
Rastegar Hoseini
Position
Assistant Professor
Latest degree
Ph.D.
Other areas of specialty/work
تغذیه ورزشی
Street address
No.9, Taqh-Bostan Ave, University Blvd, Razi University
City
Kermanshah
Province
Kermanshah
Postal code
6714414971
Phone
+98 83 3427 6505
Email
R.hoseini@razi.ac.ir
Sharing plan
Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Not applicable
Data Dictionary
Not applicable
Title and more details about the data/document
All data will be recorded in the SPSS software and will be available.
When the data will become available and for how long
Availability will start nine months after publishing all papers
To whom data/document is available
Only available for researchers in academic and scientific institutions
Under which criteria data/document could be used
All data can be used as a reference
From where data/document is obtainable
hayderihsan91@gmail.com
What processes are involved for a request to access data/document
If the explanation for data request would be convincing it will be given in 3 days.