The effect of the Rose Damascena aromatherapy on stress, anxiety and the outcome of conception in women candidate for Intracytoplasmic sperm injection (ICSI)

Determining the effect of the scent of the rose flower on stress, anxiety and the result of conception of women candidates for ICSI

The clinical trial has two experimental and control groups with parallel groups, randomized on 62 women candidates for ICSI at the stage of embryo transfer into the uterus.

This study is an open-label randomized controlled clinical trial. The sampling center is Arash Women's Comprehensive Hospital. Women will be included in the study if they have moderate and severe stress and anxiety. After the implementation of the intervention and immediately before going to the operating room, the DASS21 questionnaire will be completed in both test and re-control groups, and the level of stress and anxiety in the two groups will be compared before and after the intervention. Two weeks later, we will check the results by the B-HCG test.

Inclusion primary infertility aged 20 to 40 years infertility for at least 3 years moderate and severe stress and anxiety based on the DASS21 questionnaire. Exit Using perfume Allergy to essential oils History of migraine Disturbance in smell severe mental and physical diseases Occurrence of severe stressful factors in the last 6 months The need to use to reduce stress and anxiety Addiction to tobacco, drugs and alcohol

One hour before going to the embryo transfer room, aromatherapy and placebo are performed in the groups, and the level of stress and anxiety in the groups is compared before and after the intervention. Two weeks later, we will check the B HCG test.

1- Aromatherapy with the scent of roses and placebo on moderate or severe stress and anxiety, which is measured and compared with the DASS 21 questionnaire. 2- Pregnancy result is determined 2 weeks after aromatherapy and embryo injection

The sampling method is accessible and based on the study entry and exit criteria. In this way, after obtaining written consent from the samples, people are assigned to 2 intervention and control groups using block random allocation method and blocks of 4. Blocks are selected randomly and by placement.By using this method, it can be ensured that an equal number of participants will enter the experimental and control groups in consecutive time intervals. In order to prevent the possibility of the effect of the aroma spread in the air on the people of the studied groups, the department manager will be requested to place the studied samples in a separate room based on randomization and the same type of intervention received. For blocks of 4, six modes are considered as follows: 1. TTCC 2. TCTC 3. TCCT 4. CCTT 5. CTCT 6. CTTC