Evaluation of the effect of vasopressin injection technique in comparison to water injection technique in laparoscopic excision on ovarian reserve after surgery of unilateral ovarian endometrioses in the women with endometriosis that referred to educational hospitals of Shiraz University of Medical Sciences

Determining the effect of vasopressin injection in laparoscopic excision on ovarian reserve after unilateral ovarian endometrioma surgery.

This controlled, parallel-group, randomized, phase 3 clinical trial will be conducted on 60 women diagnosed with endometriosis and unilateral ovarian endometriosis (OMA) (with clinical and ultrasound confirmation by gynecologists). The method of random allocation of permutation blocks is done using Random Allocation software.

The researcher will select 60 patients with endometriosis referred to Faqihi Hospital in Shiraz and will randomly divide the permutation blocks into two groups A (vasopressin injection) and B (water injection). Before starting the intervention, ovarian reserve test will be done for all patients under study. Three and six months after surgery, the Anti Mullerian Hormone (AMH) is performed again in both intervention and control groups and compared with the findings of the ovarian reserve test before surgery.

Inclusion criteria 18-45-year-old woman with endometriosis with clinical and ultrasound confirmation of endometriosis specialists and unilateral OMA exclusion criteria • Patients who had more than one operation due to endometriosis • Patients with previous history of ovarian surgery • Patients with previous history of endometriosis surgery • Patients with AMH < 0.5 or FSH > 10 • History of chemotherapy and radiotherapy

In group A (intervention), before starting cystectomy, a one-time injection of 5 cc of vasopressin solution is performed in the mesovarium space with a 3.5 x 33.5 aspiration needle in the cyst wall. In group B (control), normal saline injection is performed before cystectomy

Anti Mullerian Hormone (AMH) AMH level is measured before, three and six months after surgery using ELISA test.

The method of random allocation of replacement blocks will be done using Random Allocation software. In this way, first, various quadruple blocks are created (ABBA, BABA, ABAB, BBAA, AABB, and BABA) and one of these blocks is randomly selected, and according to the order mentioned in the selected block, the patients are divided into one of two groups, A or B. be. Then randomization will be done for other patients as well. In group A (intervention), before the start of cystectomy, a one-time injection of 5 cc of vasopressin solution is performed in the mesovarium space with aspiration needle 33.5 x 3.5 in the cyst wall, and then cystectomy will be performed for them in group B (control) before At the beginning of cystectomy, normal saline injection is performed and then cystectomy will be performed for them.