Investigating the Effect of Short-Term Royal Jelly Supplementation on Muscle Damage, Fatigue, and Pain Responses in Male Athletes After Exhaustive Aerobic Exercise

The aim of the present study was to investigate the effect of short-term Royal Energy supplementation on muscle damage, fatigue, and pain response in male athletes to exhaustive aerobic exercise.

This study is a randomized, single-blind, placebo-controlled trial involving 28 male athletes aged 20 to 30 years. Participants will be randomly assigned to one of two groups: the Royal Energy supplement group (n = 14) and the placebo group (n = 14). Both groups will engage in a similar training program prior to the start of supplementation and will provide blood samples for the measurement of lactate, dehydrogenase, and creatine kinase at four-time points (before and after two bouts of activity).

Faculty of Physical Education and Sport Sciences, Razi University, Kermanshah

Inclusion Criteria Age and Gender: Participants must be adult males between the ages of 18 and 35. Health Status: Participants must be in good general health and have no chronic illnesses or serious medical problems. Exercise History: Participants must have at least a few months of experience in aerobic exercise. Exclusion Criteria Chronic Diseases: Individuals with chronic diseases such as diabetes, heart disease, or respiratory problems are not eligible to participate in the study. Allergic Sensitivities: Individuals with a history of severe food or supplement allergies, especially to royal jelly, are excluded from the study.

Royal Energy Supplement Group This group will receive short-term Royal Energy supplements for a specified period before and after exhausting aerobic activities. Control Group (Placebo) This group will receive a placebo, which is an inactive powder designed to resemble the Royal Energy supplement in both appearance and taste.

Muscle damage, fatigue, and pain response

In the present study, a simple randomization method will be employed. Each participant will be assigned a unique identification number and then allocated to one of two groups using a random number table. This revision maintains the original meaning while enhancing readability.

Blinding is a crucial aspect of clinical study design that helps minimize bias and enhance the accuracy of results. In the study titled "Investigating the Effect of Short-Term Royal Energy Supplementation on Muscle Damage, Fatigue, and Pain Response in Male Athletes to Exhaustive Aerobic Exercise," a one-sided blinding method will be utilized. Below is a detailed explanation of the blinding procedure: 1. Definition of One-Sided Blinding One-sided blinding refers to a situation where only the participants are unaware of the type of intervention they are receiving (either the Royal Energy Supplement or a placebo), while the researchers and investigators know which intervention is being administered. This approach aims to reduce the psychological effects that participants' expectations may have on the study outcomes. 2. Methods of Blinding - Use of Placebo: For the control group that does not receive the supplement, a placebo (such as an inert powder) will be used that closely resembles the actual supplement in appearance and taste. This similarity ensures that participants cannot distinguish between the real supplement and the placebo. - Training Participants: Prior to the start of the study, participants will be informed that they may receive either a supplement or a placebo but will not be told which one they are assigned to. This helps maintain the integrity of the blinding process. - Data Collection Protocols: Researchers will conduct data collection and assessments in a manner that does not reveal any information about the type of intervention to participants. This includes using independent questionnaires and assessments that are designed without reference to the intervention type. 3. Monitoring Compliance with Blinding - Continuous Monitoring: The research team will continuously monitor adherence to blinding protocols to ensure that no information is inadvertently disclosed to participants regarding their type of intervention. - Periodic Assessments: Throughout the study, assessments will be conducted to evaluate whether participants can correctly identify their intervention type. This helps gauge the effectiveness of the blinding process.

All data will be recorded in the SPSS software and will be available

Availability will start nine months after publishing all papers

Only available for researchers in academic and scientific institutions

All data can be used as a reference

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If the explanation for the data request would be convincing it will be given in 3 days.