The effect of a course of vitamin D and calcium supplementation along with squat exercises on angiogenesis indices in the elderly

The aim of the present study was to investigate the protective effect of exercise combined with calcium and vitamin D supplementation on angiogenesis indices in elderly men.

The clinical trial will be a controlled, double-blind, parallel-group trial (supplement and exercise, placebo and exercise and control) on 45 elderly people. A random number table will be used for randomization.

After selecting 45 eligible elderly men who were referred to health centers in Zabol city and grouping the individuals, for four weeks, the exercise groups will follow three exercise sessions with 5% of body weight per week, and the supplement groups will follow a program of one 1000 mg calcium tablet per day along with one 50,000 international unit vitamin D capsule per week. For double-blinding, before the start of the study, the packages containing the supplement and placebo will be marked by a person other than the researcher, and the supplement and placebo will be taken under the supervision of the same person.

Age range: 60-70 years, BMI between 18.5 and 25 kg/m2, having a general level of physical and mental health, inactive lifestyle. Exclusion criteria: using walking aids, using medication in the previous 6 months, having cardiovascular diseases

Intervention group 1: Training with supplement, one 1000 mg calcium tablet per day along with one 50,000 IU vitamin D capsule per week and squat exercises 3 sessions per week. Intervention group 2: Training with placebo, one 1000 mg placebo tablet per day along with one placebo capsule per week and squat exercises. Control group: Continuing daily activities without intervention

Vascular endothelial growth factor, Nitric oxide, Fibroblast growth factor

Randomization of numbers is done using Random Allocation software and permutation block method. People will be assigned to three groups using ternary permutation blocks. To do this, the letters A, B, and C will be considered for the two intervention and control groups, respectively. The possible states in the ternary block are six states including CAB, BCA, CBA, ACB, BAC, ABC. Each state will be written on a card. The cards will be assigned numbers one to six. Then, one of the six possible states will be selected using dice and based on that, the selected patients will be assigned to three groups. This process will continue until the sample size is completed.

Participants in the supplement group will receive 1,000 mg of calcium daily and 50,000 IU of vitamin D weekly. The placebo groups will receive the same number of starch tablets and capsules. Before the study begins, the supplement and placebo packages will be marked by someone other than the researcher so that the researcher and participants are unaware of the type of capsules they are receiving. The supplement and placebo will be administered under the supervision of the same person.

Participants' information will be made available to other researchers after being de-identified

After the publication of the final research report.

Researchers

Citing the findings by citing the source

Those interested in more information can contact through the email and address below: Mailing address: University of Zabol Email: elhamghasemi@uoz.ac.ir

Those interested in more information can contact through the email and address below: Mailing address: University of Zabol Email: elhamghasemi@uoz.ac.ir