The effect of eight weeks of HIIT trainings and probiotic supplementation on gene expression of receptors and plasma concentration of serotonin, and heart rate variability in sedentary young women

Determining the effect of eight weeks of high-intensity interval training and probiotic supplementation on serotonin receptor and transporter gene expression, serum serotonin concentration, and heart rate variability in inactive young women

A randomized, non-blinded, phase 1, controlled clinical trial with three parallel groups on thirty healthy, inactive women. Randomization will be performed using individual coding and the data sorting function in statistical software.

The present study will be conducted in the form of an experimental design in the club of the Faculty of sport sciences, in two stages: pre-test and post-test. In the pre-test stage, the subjects' body composition, a five-minute electrocardiogram recording, blood sampling, taking blood pressure, completing a mood profile questionnaire, and a running speed test will be performed. Subjects will be divided into three groups: exercise group, supplement group, and supplement + exercise group. Then, interventions that include eight weeks of high-intensity interval training and probiotic supplementation will be performed for three days a week. In the post-test stage, all the tests mentioned in the pre-test stage will be performed.

Female gender, age 20-40 years, no exercise for 6 months; no history of neurological, cardiovascular, respiratory, metabolic diseases, or orthopedic problems

The first group; high-intensity interval training, the second group; probiotic supplementation, the third group; high-intensity interval training + probiotic supplementation.

serum serotonin concentration, serotonin receptor gene expression, serotonin transporter gene expression, heart rate variability

In the present study, the stratified randomization method will be used. First, a numerical code will be assigned to each participant, and the numerical codes will be entered into the statistical software in order. After the pre-test stage, the obtained data will be recorded against the relevant codes in the statistical software and the data will be sorted from low to high values ​​using the ascending sorting icon. Finally, three subgroups (classes) of the total number of participants will be created based on body mass index (less than and equal to 25, higher than 25 and higher than 30) and some heart rate variability indices such as the ratio of high-frequency waves to low-frequency waves of the electrocardiogram (less than one, more than 2, between one and two), and from each subgroup (class), an equal number of participants will enter the main group in the form of numerical and anonymous codes. In this way, the division of groups is done in a homogeneous but random manner. For the purpose of allocation concealment, the numerical codes will not be identified by the researcher during grouping.

A file related to part of the participants' heart rate variability data, a file related to part of the blood biochemical tests, genetics, and the informed consent form will be prepared as an attachment and will be sent upon request.

The access period will begin 6 months after the results are published.

Data will be available for researchers working at universities and scientific institutions.

The documentation will be available for use by medical staff, especially neurologists or physiologists working in clubs, and will be made available to them upon request, subject to review of the conditions.

To receive the data file, applicants can contact Ms. Noushin Zakeri at the email address noushin.zakeri.82@gmail.com.

The applicant must provide documentation indicating the name of the university or scientific institution in which he/she is working and state his/her clear purpose for requesting raw data.