History
# Registration date Revision Id
2 2019-08-30, 1398/06/08 102463
1 2018-12-05, 1397/09/14 69651
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  • Protocol summary

    Population and sample size: Among patients with CHD referred to Naghavi Clinic affiliated to Kashan University of Medical Sciences, 60 patients will be selected according to inclusion and exclusion criteria.
    Population and sample size: Among patients with CHD referred to Naghavi outpatient Clinic affiliated to Kashan University of Medical Sciences, 60 patients will be selected according to inclusion and exclusion criteria.
    از بین بیماران مبتلا به بیماری کرونر قلبی ارجاع شده به کلینیک نقوی وابسته به دانشگاه علوم پزشکی کاشان، 60 بیمار بر اساس معیار های ورود و خروج از مطالعه انتخاب خواهند شد.
    از بین بیماران مبتلا به بیماری کرونر قلبی ارجاع شده به کلینیک سرپایی نقوی وابسته به دانشگاه علوم پزشکی کاشان، 60 بیمار بر اساس معیار های ورود و خروج از مطالعه انتخاب خواهند شد.
    Individuals aged 40-75 years diagnosed with type 2 diabetes and CHD will be included in this study. Exclusion criteria: Unwillingness to cooperate
    patients with T2DM, aged 40-85 years old with proven 2- and 3-vessel CHD will be included in this study. Exclusion criteria: consuming resveratrol three months prior to the intervention, taking antioxidant and/or anti-inflammatory supplements such as vitamin E and vitamin C, having an acute myocardial infarction or cardiac surgery in the past three months, or having renal or hepatic failure
    معیار ورود به مطالعه: افراد 75-40 ساله مبتلا به بیماری دیابت تیپ 2 و کرونر قلبی. معیار خروج از مطالعه: عدم تمایل به همکاری
    معیار ورود به مطالعه: افراد 85-40 ساله مبتلا به بیماری دیابت تیپ 2 و کرونر قلبی با گرفتگی در دو یا سه رگ. معیار خروج از مطالعه: مصرف رزوراترول طی سه ماه قبل از شروع مداخله، مصرف مکمل های آنتی اکسیدان و ضد التهابی از قبیل ویتامین E و ویتامین C، داشتن سابقه نارسایی حاد قلبی ویا جراحی قلب طی سه ماه گذشته، ابتلا به نارسایی کلیوی و کبدی
    Insulin metabolism parameters (primary outcomes) and biomarkers of inflammation and oxidative stress factors (secondry outcomes) will be quantified at study baseline and end-of-trial.
    Insulin resistance (primary outcomes) and biomarkers of inflammation and oxidative stress factors, FPG, insulin, QUICKI, serum lipids, IL-1, PPAR-γ, TNF-α, TGF-β, SIRT1 (secondry outcomes) will be quantified at study baseline and end-of-trial.
    مارکر های متابولیسم انسولین(پیامدهای اولیه)، بیومارکر های التهابی و فاکتور های استرس اکسیداتیو (پیامدهای ثانویه) در ابتدا و انتهای مداخله اندازه گیری خواهد شد.
    مقاومت به انسولین (پیامدهای اولیه)، بیومارکر های التهابی و فاکتور های استرس اکسیداتیو، قند خون ناشتا، انسولین، شاخص حساسیت به انسولین، لیپیدهای سرم، اینترلوکین1، گیرنده های فعال کننده تکثیر پروکسی زوم ها، فاکتور نکروز دهنده تومور آلفا، فاکتور رشد تغییر دهنده بتا، سیرتوئین (پیامدهای ثانویه) در ابتدا و انتهای مداخله اندازه گیری خواهد شد.
  • General information

    75
    85
    2018-09-01, 1397/06/10
    2018-09-16, 1397/06/25
    Subjects aged 40-75 years; diagnosed with type 2 diabetes and CHD
    Not having history of inflammatory or autoimmune diseases, malignancies, thyroid
    patients with T2DM, aged 40-85 years old with proven 2- and 3-vessel CHD
    افراد 75-40 ساله مبتلا به بیماری دیابت تیپ 2 و کرونر قلبی
    نداشتن بیماری‌های خاص سرطان، بیماریهای التهابی، اتوایمیون و هایپر یا هایپوتیروئیدی
    افراد 85-40 ساله مبتلا به بیماری دیابت تیپ 2 و کرونر قلبی با گرفتگی در دو یا سه رگ
    Unwillingness to cooperate
    Current acute infection or inflammatory disease like rheumatoid arthritis
    Diagnosis of renal failure
    Diagnosis of Chronic Liver Disease
    Consuming resveratrol three months prior to the intervention
    Taking antioxidant and/or anti-inflammatory supplements such as vitamin E and vitamin C
    Having an acute myocardial infarction or cardiac surgery in the past three months
    Having renal or hepatic failure
    عدم تمایل به همکاری
    ابتلا به عفونت حاد یا بیماریهای التهابی مزمن نظیر روماتوئیدآرتریت
    ابتلا به نارسایی کلیوی
    ابتلا به بیماری مزمن کبدی
    مصرف رزوراترول طی سه ماه قبل از شروع مداخله
    مصرف مکمل های آنتی اکسیدان و ضد التهابی از قبیل ویتامین E و ویتامین C
    داشتن سابقه نارسایی حاد قلبی و یا جراحی قلب طی سه ماه گذشته
    ابتلا به نارسایی کلیوی و کبدی
    randomization will be performed with simple method and random numbers generated by computer software.
    Randomization will be performed with balanced randomization method and random numbers generated by computer software.
    نحوه تصادفی سازی با روش ساده و استفاده از اعداد تصادفی ایجاد شده توسط نرم افزار کامپیوتری انجام خواهد شد
    نحوه تصادفی سازی با روش تعادلی و استفاده از اعداد تصادفی ایجاد شده توسط نرم افزار کامپیوتری انجام خواهد شد
  • Secondary outcomes

    #1
    empty
    Gene expression of sirtuin1
    empty
    بیان ژن سیرتوئین
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Real Time PCR
    empty
    ریل تایم پی سی آر
    #2
    empty
    Gene expression of interleukin-1(IL-1)
    empty
    بیان ژن اینترلوکین -1
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Real Time PCR
    empty
    ریل تایم پی سی آر
    #3
    empty
    Gene expression of tumor necrosis factor alpha (TNF-α)
    empty
    بیان ژن TNF-α
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Real Time PCR
    empty
    ریل تایم پی سی آر
    #4
    empty
    Gene expression of transforming growth factor beta (TGF-β)
    empty
    بیان ژن TGF-β
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Real Time PCR
    empty
    ریل تایم پی سی آر
    #5
    empty
    Gene expression of peroxisome proliferator-activated receptor gamma (PPAR-γ)
    empty
    بیان ژن PPAR-γ
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Real Time PCR
    empty
    ریل تایم پی سی آر
    #6
    empty
    Fasting plasma glucose
    empty
    قند خون ناشتا
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #7
    empty
    Insulin
    empty
    انسولین
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Elisa kit
    empty
    کیت الایزا
    #8
    empty
    Triglycerides
    empty
    تری گلیسرید
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #9
    empty
    VLDL-cholesterol
    empty
    VLDL کلسترول
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #10
    empty
    Total cholesterol
    empty
    کلسترول تام
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #11
    empty
    LDL-cholesterol
    empty
    LDL کلسترول
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #12
    empty
    HDL-cholesterol
    empty
    HDL کلسترول
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #13
    empty
    Quantitative insulin sensitivity check index(QUICKI)
    empty
    شاخص حساسیت به انسولین
    empty
    At the beginning of the study and after 4 weeks of intervention
    empty
    در ابتدای مطالعه و 4 هفته بعد از مداخله
    empty
    Calculation using standard formula
    empty
    استفاده از فرمول های استاندارد
  • Recruitment centers

    #1
    Name of recruitment center - English: Naghavi Clinic
    Name of recruitment center - Persian: کلینیک نقوی
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Street address - English: Shahid Rajaee Avenue, Kashan
    Street address - Persian: کاشان، خیابان شهید رجایی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Country: Iran (Islamic Republic of)
    Postal code: 8713743444
    Phone: +98 31 5545 0031
    Fax:
    Email: naghavihospital@kaums.ac.ir
    Web page address:
    Name of recruitment center - English: Naghavi outpatient Clinic
    Name of recruitment center - Persian: کلینیک سرپایی نقوی
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Street address - English: Shahid Rajaee Avenue, Kashan
    Street address - Persian: کاشان، خیابان شهید رجایی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Country: Iran (Islamic Republic of)
    Postal code: 8713743444
    Phone: +98 31 5545 0031
    Fax:
    Email: naghavihospital@kaums.ac.ir
    Web page address:

Protocol summary

Study aim
The aim of this study is to determine the effects of Resveratrol supplementation on metabolic profilesand and genes expression of Sirtuin-1 ،TNF-α، TGF-β، IL-1، PPARγ in diabetetic coronary artery disease patients
Design
Parallel double-blind (both patients and researchers) clinical trial. Randomization will be done by the use of computer-generated random numbers.
Settings and conduct
Population and sample size: Among patients with CHD referred to Naghavi outpatient Clinic affiliated to Kashan University of Medical Sciences, 60 patients will be selected according to inclusion and exclusion criteria.
Participants/Inclusion and exclusion criteria
patients with T2DM, aged 40-85 years old with proven 2- and 3-vessel CHD will be included in this study. Exclusion criteria: consuming resveratrol three months prior to the intervention, taking antioxidant and/or anti-inflammatory supplements such as vitamin E and vitamin C, having an acute myocardial infarction or cardiac surgery in the past three months, or having renal or hepatic failure
Intervention groups
Patients will be assigned into two groups to receive Resveratrol supplement (n=30) or placebo (n=30). Resveratrol and placebo capsules are similar in shape and size. Fasting blood samples will be taken at baseline and 4 weeks after the intervention. At the beginning and end of the intervention: 4 weeks.
Main outcome variables
Insulin resistance (primary outcomes) and biomarkers of inflammation and oxidative stress factors, FPG, insulin, QUICKI, serum lipids, IL-1, PPAR-γ, TNF-α, TGF-β, SIRT1 (secondry outcomes) will be quantified at study baseline and end-of-trial.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20181029041490N1
Registration date: 2018-12-05, 1397/09/14
Registration timing: retrospective

Last update: 2019-09-04, 1398/06/13
Update count: 1
Registration date
2018-12-05, 1397/09/14
Registrant information
Name
Asma Hoseini
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 31 5533 8702
Email address
hosseini-as@kaums.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2018-08-16, 1397/05/25
Expected recruitment end date
2018-09-16, 1397/06/25
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of Resveratrol supplement on metabolic profiles and genes expression of Sirtuin-1، TNF-α، TGF-β، IL-1، PPARγ in diabetetic coronary artery disease patients
Public title
Effect of supplementation in treatment of Coronary Heart Disease
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
patients with T2DM, aged 40-85 years old with proven 2- and 3-vessel CHD
Exclusion criteria:
Consuming resveratrol three months prior to the intervention Taking antioxidant and/or anti-inflammatory supplements such as vitamin E and vitamin C Having an acute myocardial infarction or cardiac surgery in the past three months Having renal or hepatic failure
Age
From 40 years old to 85 years old
Gender
Both
Phase
2
Groups that have been masked
  • Participant
  • Care provider
  • Investigator
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
Randomization will be performed with balanced randomization method and random numbers generated by computer software.
Blinding (investigator's opinion)
Double blinded
Blinding description
In this study, participants, researchers, data collectors, evaluators and data analyzers are not aware of which group is placebo or supplemented.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Kashan University of Medical Sciences
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
8715981151
Approval date
2018-08-13, 1397/05/22
Ethics committee reference number
IR.Kaums.MEDNT.REC.1397.41

Health conditions studied

1

Description of health condition studied
Coronary Heart Disease
ICD-10 code
I25.9
ICD-10 code description
Chronic ischemic heart disease, unspecified

Primary outcomes

1

Description
Insulin resistance
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Calculation using HOMA formula

Secondary outcomes

1

Description
hs-CRP
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Elisa kit

2

Description
Total antioxidant
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Spectrophotometry

3

Description
Malondialdehyde
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Spectrophotometry

4

Description
Glutathione
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Spectrophotometry

5

Description
Gene expression of sirtuin1
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Real Time PCR

6

Description
Gene expression of interleukin-1(IL-1)
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Real Time PCR

7

Description
Gene expression of tumor necrosis factor alpha (TNF-α)
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Real Time PCR

8

Description
Gene expression of transforming growth factor beta (TGF-β)
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Real Time PCR

9

Description
Gene expression of peroxisome proliferator-activated receptor gamma (PPAR-γ)
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Real Time PCR

10

Description
Fasting plasma glucose
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Enzymatic kit

11

Description
Insulin
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Elisa kit

12

Description
Triglycerides
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Enzymatic kit

13

Description
VLDL-cholesterol
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Enzymatic kit

14

Description
Total cholesterol
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Enzymatic kit

15

Description
LDL-cholesterol
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Enzymatic kit

16

Description
HDL-cholesterol
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Enzymatic kit

17

Description
Quantitative insulin sensitivity check index(QUICKI)
Timepoint
At the beginning of the study and after 4 weeks of intervention
Method of measurement
Calculation using standard formula

Intervention groups

1

Description
Intervention group: Resveratrol supplements, 500mg, daily, for 4 weeks orally.
Category
Treatment - Drugs

2

Description
Control group: Placebo, daily, for 4 weeks orally.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Naghavi outpatient Clinic
Full name of responsible person
Zatollah Asemi
Street address
Shahid Rajaee Avenue, Kashan
City
Kashan
Province
Isfehan
Postal code
8713743444
Phone
+98 31 5545 0031
Email
naghavihospital@kaums.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Gholamali Hamidi
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5554 0021
Email
research@kaums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Kashan University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Gholamreza Namazi
Position
Academic staff member of the Department of Clinical Biochemistry
Latest degree
Ph.D.
Other areas of specialty/work
Biochemistry
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5554 0021
Email
namazimajid@gmail.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Gholamreza Namazi
Position
Academic staff member of the Department of Clinical Biochemistry
Latest degree
Ph.D.
Other areas of specialty/work
Biochemistry
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5554 0021
Email
namazimajid@gmail.com

Person responsible for updating data

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Gholamreza Namazi
Position
Academic staff member of the Department of Clinical Biochemistry
Latest degree
Ph.D.
Other areas of specialty/work
Biochemistry
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5554 0021
Email
namazimajid@gmail.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
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